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KMID : 0545120000100020187
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 2 p.187 ~ p.194
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Production and Characterization of a Novel Microbial Transglutaminase from Actinomadura sp.T-2
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Kim, Hyun Soo
Jung, Sang Hoon/Lee, In Seon/Yu, Tae Shick
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Abstract
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An actinomycetes strain, T-2, which produces transglutaminase (EC 2.3.2.13), was isolated from soil and identified as belonging to the Actinomadura sp., based on taxonomic studies. The conditions for the transglutaminase production and its enzymatic properties were investigated. The optimum components for the transglutaminase production were 2% glucose, 1% polypeptone and soytone, and 0.1% MnCl_2. The optimum pH and temperature of the enzyme reaction were pH 8.0 and 45℃, respectively. The enzyme was stable within the pH range of 5.0-9.0 and 30℃-45℃. The novel enzyme required no calcium ions for its activity. This enzyme polymerized various proteins such as casien, soy protein, hemoglobin, egg white, gelatin, and soybean milk.
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